Recombinant Rat Aqp4 protein, His & GST-tagged

• Cat.No.: Aqp4-2602R
• Product Overview: Recombinant Rat Aqp4 aa. (Asp179~Asp314) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: GST&His
• Protein Length: Asp179~Asp314
• Description: This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
• Form: Freeze-dried powder
• Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.2.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Aqp4 aquaporin 4 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Aqp4
• Synonyms: Aqp4; aquaporin 4; Miwc; WCH4; AQP-4; aquaporin-4; mercurial-insensitive water channel
• Gene ID: 25293
• mRNA Refseq: NM_001270558.2
• Protein Refseq: NP_001257487.1
• UniProt ID: P47863