Recombinant Rat Apoe Protein, His-tagged

• Cat.No.: Apoe-228R
• Product Overview: Recombinant Rat Apoe Protein (Glu19-Arg168) with N-His tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Glu19-Arg168
• Description: Enables several functions, including amyloid-beta binding activity; hydroxyapatite binding activity; and phospholipid binding activity. Involved in several processes, including cellular response to alcohol; neurogenesis; and regulation of lipid metabolic process. Located in several cellular components, including late endosome; neuronal cell body; and nuclear envelope. Is extrinsic component of external side of plasma membrane. Part of several cellular components, including discoidal high-density lipoprotein particle; low-density lipoprotein particle; and triglyceride-rich plasma lipoprotein particle. Colocalizes with early endosome and microtubule. Used to study artery disease (multiple); familial hyperlipidemia; fatty liver disease (multiple); glomerulosclerosis; and middle cerebral artery infarction. Biomarker of several diseases, including glomerulonephritis (multiple); hyperhomocysteinemia; hypertension; sciatic neuropathy; and transient cerebral ischemia. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease (multiple); artery disease (multiple); biliary tract cancer (multiple); eye disease (multiple); and familial hyperlipidemia (multiple). Orthologous to human APOE (apolipoprotein E).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.3 kDa
  Accurate Molecular Mass: \
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Apoe apolipoprotein E [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Apoe
• Synonyms: APOE; apolipoprotein E; apo-E; APOEA
• Gene ID: 25728
• mRNA Refseq: NM_138828
• Protein Refseq: NP_620183
• UniProt ID: P02650