Recombinant ProMatrix Metalloproteinase-7

• Cat.No.: MMP7-94
• Product Overview: Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Physical Appearance: Sterile clear liquid solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein (1 mg/ml) contains the following additives 25 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5 mM CaCl₂, 0.01% Brij-35 and 0.02% NaN3.
• Biological Activity: The specific activity was found to be 1400 IU/mg.
• Unit Definition: One unit is defined as the digestion of 1 µg Azocoll/min at 37°C.
• Stability: ProMMP-7 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Synonyms: Matrilysin; EC 3.4.24.23; MMP-7; MMP7; Matrin; MPSL1; PUMP-1; PUMP1; matrin; Pump-1 protease; Uterine metalloproteinase; Matrix metalloproteinase-7; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7; matrix metalloproteinase 7 (matrilysin, uterine); uterine matrilysin; matrilysin, matrin; uterine metalloendopeptidase; putative (or punctuated) metalloproteinase-1; matrix metalloproteinase pump 1; MMP 7; PUMP-1 proteinase; PUMP; metalloproteinase pump-1; putative metalloproteinase; MMP.