Active Recombinant M.viridifaciens neuraminidase NA Protein, His-tagged

• Cat.No.: NA-474M
• Product Overview: Recombinant M.viridifaciens neuraminidase (BAA00852.1) (Pro42-Gly403) was expressed, with a N-terminal polyhistidine tag

Specification

• Species: M.viridifaciens
• Source: E.coli
• Tag: His
• Protein Length: Pro42-Gly403
• Predicted N Terminal: Met 1
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is 10,000 pmoles/min/μg. as measured under the described conditions.
• Molecular Mass: The recombinant M.viridifaciens neuraminidase consisting of 369 amino acids and has a calculated molecular mass of 39.7 kDa as estimated in SDS-PAGE under reducing conditions as predicted.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: NA
• Synonyms: neuraminidase;NA Protein