Recombinant Mouse YWHAE, GST-tagged
Cat.No. : | YWHAE-1193M |
Product Overview : | A DNA sequence encoding the mouse YWHAE ( P62259 ) ( Met 1 – Gln 255 ) was fused with the GST tag at the N-terminus |
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Species : | Mouse |
Source : | Human |
Tag : | His |
Protein Length : | 1-255 a.a. |
Description : | Mouse tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, epsilon polypeptide ( YWHAE ), is a member of the14- 3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. |
Form : | Lyophilized from sterile 50 mM Tris, 2 mM GSH, 1 mM DTT, pH 8.2 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Purity : | > 90 % as determined by SDS-PAGE |
Endotoxin : | ﹤1.0 EU per μg protein as determined by the LAL method |
Stability : | Samples are stable for up to twelve months from date of receipt at -70°C |
Predicted N terminal : | Met |
Molecular Mass : | The recombinant mouse YWHAE / GST chimera consists of 487 amino acids and has a predicted molecular mass of 56.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions |
Storage : | Store it under sterile conditions at -70°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
Pathways : | Alpha6-Beta4 Integrin Signaling Pathway; Calcium Regulation in the Cardiac Cell; Centrosome maturation |
Gene Name | Ywhae tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide [ Mus musculus ] |
Official Symbol | Ywhae |
Synonyms | Ywhae; AU019196; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide;14-3-3 protein epsilon; 14-3-3E; 14-3-3 epsilon |
Gene ID | 22627 |
mRNA Refseq | NM_009536 |
Protein Refseq | NP_033562 |
UniProt ID | Q5SS40 |
Chromosome Location | 11 B2 |
Function | enzyme binding; histone deacetylase binding; monooxygenase activity |
◆ Recombinant Proteins | ||
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YWHAE-888M | Recombinant Mouse YWHAE protein(Met1-Gln255), GST-tagged | +Inquiry |
YWHAE-1193M | Recombinant Mouse YWHAE, GST-tagged | +Inquiry |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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