Recombinant Mouse Xrcc2 Protein, Myc/DDK-tagged

• Cat.No.: Xrcc2-7023M
• Product Overview: Purified recombinant protein of Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
• Molecular Mass: 31.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Xrcc2 X-ray repair complementing defective repair in Chinese hamster cells 2 [ Mus musculus (house mouse) ]
• Official Symbol: Xrcc2
• Synonyms: XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; DNA repair protein XRCC2; recA/RAD51 family protein; X-ray repair cross-complementing protein 2; RecA; RAD51; 4921524O04Rik; 8030409M04Rik
• Gene ID: 57434
• mRNA Refseq: NM_020570
• Protein Refseq: NP_065595
• UniProt ID: Q9CX47