Recombinant Mouse Wingless-related MMTV Integration Site 3A

• Cat.No.: Wnt3a-1788M
• Product Overview: A DNA sequence encoding the signal peptide from human CD33, Met 1 - Ala 16, joined with the mature mouse Wnt-3A, Ser 19 - Lys 352 was inserted into a suitable mammalian expression vector and expressed inCHOcells.

Specification

• Cat. No.: Wnt3a-1788M
• Description: Wingless-type MMTV integration site family, member 3A, also known as WNT3A is an intercellular signaling molecule. WNT3A is expressed extensively in cells fated to give rise to embryonic mesoderm. Wnt-3a regulates survival, expansion, and maintenance of neural progenitors derived from embryonic stem cells. It has been reported that Wnt-3a supports self-renewal of embryonic stem cells and can maintain these cells in a pluripotent state. Wnt-3a is also considered a ligand that activates the beta-catenin-dependent pathway in Wnt signaling, which is implicated in numerous physiological events such as morphogenesis.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse WNT-3a is Mr 37 kDa. However, because of glycosylation the actual molecular weight as observed by migration on SDS Page is Mr 41 kDa.
• Source: CHO.
• State Of Matter: Lyophilized.
• Purity: >75% as determined by SDS-PAGE and visualized by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Mouse WNT-3a was determined by its ability to synergize with BMP-2 to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells. The expected ED50 is typically 2 - 10 ng/mL, in the presence of a low or non-inducing amount of recombinant human BMP-2 (5 - 15 ng/mL). Approximately 50 - 100 ng/mL results in maximal synergy with BMP-2 activity.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
• Tag: Non
• Protein length: 19-352 a.a.

Gene Information

• Gene Name: Wnt3a wingless-related MMTV integration site 3A [ Mus musculus ]
• Synonyms: Wnt3a; wingless-related MMTV integration site 3A; vt; Wnt-3a; vestigial tail; OTTMUSP00000005961
• Gene ID: 22416
• mRNA Refseq: NM_009522
• Protein Refseq: NP_033548
• UniProt ID: P27467
• Chromosome Location: 11 32.0 cM
• Pathway: Basal cell carcinoma; Hedgehog signaling pathway; Melanogenesis; Pathways in cancer; Wnt signaling pathway
• Function: receptor binding; protein binding; signal transducer activity