Recombinant Mouse Ube2l3 Protein, Myc/DDK-tagged

• Cat.No.: Ube2l3-6792M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2L 3 (Ube2l3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
• Molecular Mass: 18.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2l3 ubiquitin-conjugating enzyme E2L 3 [ Mus musculus (house mouse) ]
• Official Symbol: Ube2l3
• Synonyms: UBE2L3; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme E2 L3; ubiquitin-protein ligase L3; ubiquitin carrier protein L3; ubiquitin-conjugating enzyme 7; UbcM4; Ubce7; C79827; MGC118100
• Gene ID: 22195
• mRNA Refseq: NM_009456
• Protein Refseq: NP_033482
• UniProt ID: P68037