Recombinant Mouse Twist1 Protein, Myc/DDK-tagged

• Cat.No.: Twist1-6740M
• Product Overview: Purified recombinant protein of mouse full-length twist basic helix-loop-helix transcription factor 1 (Twist1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. This gene encodes a bHLH transcription factor that is evolutionarily conserved from invertebrates to humans, and was originally identified in Drosophila as an essential gene involved in early mesoderm development and dorsal-ventral patterning in the embryo. This protein plays a role in cancer by regulating the epithelial-mesenchymal transition (EMT), a process that is critical for metastasis initiation, and promoting tumor progression. Mutations in the human gene are associated with Saethre-Chotzen syndrome (SCS). Mice with heterozygous mutations in this gene exhibit cranofacial and structural defects similar to those seen in human SCS patients. [provided by RefSeq, Sep 2015]
• Molecular Mass: 21.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Twist1 twist basic helix-loop-helix transcription factor 1 [ Mus musculus (house mouse) ]
• Official Symbol: Twist1
• Synonyms: TWIST1; twist homolog 1 (Drosophila); twist-related protein 1; Pluridigite; charlie chaplin; polydactyly EMS; Ska< sup> m10Jus< /sup> twist gene homolog 1; Pde; pdt; Ska10; Twist; M-Twist; bHLHa38; AA960487; m10Jus> MGC103391
• Gene ID: 22160
• mRNA Refseq: NM_011658
• Protein Refseq: NP_035788
• UniProt ID: P26687