Recombinant Mouse Ttr protein, His-tagged

• Cat.No.: Ttr-729M
• Product Overview: Recombinant Mouse Ttr aa. (Gly21~Asn147) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions
• Protein length: Gly21~Asn147
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.3.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Ttr transthyretin [ Mus musculus (house mouse) ]
• Official Symbol: Ttr
• Synonyms: Ttr; transthyretin; D17860; AA408768; AI787086; prealbumin
• Gene ID: 22139
• mRNA Refseq: NM_013697.5
• Protein Refseq: NP_038725.1
• UniProt ID: P07309