Recombinant Mouse Trmt112 Protein, Myc/DDK-tagged

• Cat.No.: Trmt112-1350M
• Product Overview: Purified recombinant protein of mouse full-length RIKEN cDNA 0610038D11 gene (cDNA clone MGC:30341 IMAGE:4162006), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase BUD23, methylates the N7 position of a guanine in 18S rRNA. The heterodimer with HEMK2/N6AMT1 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production. Together with methyltransferase METTL5, specifically methylates the 6th position of adenine in position 1832 of 18S rRNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 12.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trmt112 tRNA methyltransferase 11-2 [ Mus musculus (house mouse) ]
• Official Symbol: Trmt112
• Synonyms: Trmt112; tRNA methyltransferase 11-2; Trm11; Trm112p; 0610038D11Rik; multifunctional methyltransferase subunit TRM112-like protein; TRM112-like protein; tRNA methyltransferase 11-2 homolog; tRNA methyltransferase 112 homolog
• Gene ID: 67674
• mRNA Refseq: NM_026306
• Protein Refseq: NP_080582
• UniProt ID: Q9DCG9