Recombinant Mouse Trim25 Protein, Myc/DDK-tagged

• Cat.No.: Trim25-6647M
• Product Overview: Purified recombinant protein of mouse full-length tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.
• Molecular Mass: 70.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trim25 tripartite motif-containing 25 [ Mus musculus (house mouse) ]
• Official Symbol: Trim25
• Synonyms: Trim25; tripartite motif-containing 25; EFP; Zfp14; Zfp147; AA960166; AL022677; E3 ubiquitin/ISG15 ligase TRIM25; RING-type E3 ubiquitin transferase TRIM25; estrogen-responsive finger protein; tripartite motif protein 25; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147; EC 2.3.2.27; EC 6.3.2.n3
• Gene ID: 217069
• mRNA Refseq: NM_009546
• Protein Refseq: NP_033572
• UniProt ID: Q61510