Recombinant Mouse Top1 Protein, His-tagged

Cat.No. : Top1-465M
Product Overview : Recombinant Mouse Top1 Protein (Met1-Ser252) with N-His tag was expressed in E. coli.
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Species : Mouse
Source : E.coli
Tag : His
Protein Length : Met1-Ser252
Description : Enables DNA topoisomerase type I (single strand cut, ATP-independent) activity. Acts upstream of or within DNA replication; DNA topological change; and embryonic cleavage. Located in cytoplasm; nucleus; and perikaryon. Is expressed in brain; cerebral cortex ventricular layer; cortical plate; and telencephalon. Orthologous to human TOP1 (DNA topoisomerase I).
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 34.1 kDa
Accurate Molecular Mass: 34 kDa
Purity : > 97%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name Top1 topoisomerase (DNA) I [ Mus musculus (house mouse) ]
Official Symbol Top1
Synonyms TOP1; topoisomerase (DNA) I; DNA topoisomerase 1; DNA topoisomerase I; Top-1; AI467334; D130064I21Rik
Gene ID 21969
mRNA Refseq NM_009408
Protein Refseq NP_033434

Not For Human Consumption!

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