Recombinant Human TOP1 293 Cell Lysate

Cat.No. : TOP1-867HCL
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Species : Human
Source : HEK293
Tag : Non
Description : Antigen standard for topoisomerase (DNA) I (TOP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name TOP1 topoisomerase (DNA) I [ Homo sapiens ]
Official Symbol TOP1
Synonyms TOP1; topoisomerase (DNA) I; DNA topoisomerase 1; type I DNA topoisomerase; TOPI;
Gene ID 7150
mRNA Refseq NM_003286
Protein Refseq NP_003277
MIM 126420
UniProt ID P11387
Chromosome Location 20q12-q13.1
Pathway Caspase cascade in apoptosis, organism-specific biosystem;

Not For Human Consumption!

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