Recombinant Mouse Tbx5 Protein, His-tagged

• Cat.No.: Tbx5-2116M
• Product Overview: Recombinant Mouse Tbx5 Protein (Met51-Gln251) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Met51-Gln251
• Description: Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in heart development; positive regulation of gap junction assembly; and positive regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including cell communication involved in cardiac conduction; heart development; and positive regulation of cell population proliferation. Located in nucleus. Part of transcription regulator complex. Is expressed in several structures, including embryo mesenchyme; eye; genitourinary system; heart; and vena cava. Used to study Holt-Oram syndrome. Human ortholog(s) of this gene implicated in Holt-Oram syndrome. Orthologous to human TBX5 (T-box transcription factor 5).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.9 kDa
  Accurate Molecular Mass: 27 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Tbx5 T-box 5 [ Mus musculus (house mouse) ]
• Official Symbol: Tbx5
• Synonyms: TBX5; T-box 5; T-box transcription factor TBX5; T-box protein 5
• Gene ID: 21388
• mRNA Refseq: NM_011537
• Protein Refseq: NP_035667
• UniProt ID: P70326