Recombinant Mouse Selectin, Lymphocyte, Fc-His

• Cat.No.: Sell-1764M
• Product Overview: The extracellular doman of mouse L-selectin was fused to the carboxy-terminal 6X his-tagged Fc region of human IgG1 via a polypeptide linker inserted into a suitable mammalian expression vector and expressed inNS0 cells.

Specification

• Cat. No.: Sell-1764M
• Description: L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse L-Selectin is Mr 60.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 150 kDa.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Endotoxin: <0.1 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Mouse L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells. When 1 x 10⁴cells/well are added to L-Selectin coated plates approximately 90 - 100% will adhere for 1 hour incubation at RT.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
• Tag: His&Fc

Gene Information

• Gene Name: Sell selectin, lymphocyte [ Mus musculus ]
• Synonyms: Sell; selectin, lymphocyte; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin; selectin L
• Gene ID: 20343
• mRNA Refseq: NM_001164059
• Protein Refseq: NP_001157531
• UniProt ID: P18337
• Chromosome Location: 86.6 cM
• Pathway: Cell adhesion molecules (CAMs)
• Function: Binding; protein binding; sugar binding