Recombinant Mouse Selectin, Lymphocyte, Fc-His

• Cat.No.: Sell-661M
• Product Overview: Recombinant Mouse Selectinencoding the extracellular domain (Met 1- Asn 332) of mouse SELL (NP_035476.1) precursor was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus. It was produced inHEK293.

Specification

• Cat. No.: Sell-661M
• Description: L-selectin (SELL), also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups and are involved in the regulation of leukocyte traffic. Selectins are composed of an N-terminal C-type lectin domain, a single EGF domain, a variable number of short consensus repeat (SCR) domains, a transmembrane region and a cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin is commonly found on the cell surfaces of T cells.
• Molecular Mass: The recombinant mouse SELL/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 542 amino acids and has a predicted molecular mass of 61 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm SELL/Fc monomer is approximately 100-110 kDa due to glycosylation.
• Source: Human Cells (HEK293).
• Predicted N Terminal: Trp 39.
• Endotoxin: < 1.0 EU per 1g cytokine as determined by the LAL method.
• Formulation: Supplied as a 0.2m filtered solution of PBS, pH7.4.
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4 before opening to recover the entire contents.
• Purity: > 97%, as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt -70°C.
• Storage: Store it under sterile conditions at -70°Cimmediately after receipt. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Tag: His&Fc
• Protein length: 1-332 a.a.

Gene Information

• Gene Name: Sell selectin, lymphocyte [ Mus musculus ]
• Synonyms: Sell; selectin, lymphocyte; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin;selectin L
• Gene ID: 20343
• mRNA Refseq: NM_001164059
• Protein Refseq: NP_001157531
• UniProt ID: P18337
• Chromosome Location: 86.6 cM
• Pathway: Cell adhesion molecules (CAMs)
• Function: Binding; protein binding; sugar binding