Recombinant Mouse scavenger receptor class B, member 2, His-tagged

• Cat.No.: Scarb2-851M
• Product Overview: Recombinant Mouse Scarb2 encoding the human PLAU (NP_002649.1) (Met 1-Leu 431) with a C-terminal his tag was expressed inHEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-431 a.a.
• Description: Plasminogen activator, urokinase, also known as PLAU and UPA, is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. PLAU is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. A specific polymorphism in PLAU gene is implicated in late-onset Alzheimer disease and also with decreased affinity for fibrin-binding.
• Molecular Mass: The secreted recombinant human PLAU comprises 422 amino acids with a predicted molecular mass of 46 kDa. As a result of glycosylation, rhPLAU migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length PLAU with the molecular mass of 18, 32 and 50 kDa respectively in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 21.
• Endotoxin: < 1.0 EU per 1µg cytokine as determined by the LAL method.
• Formulation: Supplied as a 0.2µm filtered solution of PBS, pH7.4.
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Purity: > 97%, as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt -70°C.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Scarb2 scavenger receptor class B, member 2 [ Mus musculus]
• Synonyms: Scarb2; scavenger receptor class B, member 2; LGP85; Cd36l2; LIMP-2; MLGP85; 9330185J12Rik;LIMP II; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2
• Gene ID: 12492
• mRNA Refseq: NM_007644
• Protein Refseq: NP_031670
• UniProt ID: O35114
• Chromosome Location: 5
• Pathway: Lysosome
• Function: protein binding; receptor activity