Recombinant Mouse S100a5, His-tagged

• Cat.No.: S100a5-15850M
• Product Overview: Recombinant mouse S100A5 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. It is expressed in very restricted regions of the adult brain.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 40% glycerol, 3mM DTT, 200mM NaCl
• Molecular Mass: 13.4kDa (117aa), confirmed by MALDI-TOF
• Protein length: 1-93aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMETPLE KALTTMVTTF HKYSGREGSK LTLSRKELKE LIKTELSLAE KMKESSIDNL MKSLDKNSDQ EIDFKEYSVF LTTLCMAYND FFLEDNK
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: S100a5 S100 calcium binding protein A5 [ Mus musculus ]
• Official Symbol: S100A5
• Synonyms: S100A5; S100 calcium binding protein A5; protein S100-A5; S100 calcium-binding protein A5; S100D9
• Gene ID: 20199
• mRNA Refseq: NM_011312
• Protein Refseq: NP_035442
• Function: calcium ion binding; copper ion binding; metal ion binding; protein homodimerization activity; zinc ion binding