Recombinant Mouse Rybp Protein, Myc/DDK-tagged

• Cat.No.: Rybp-5662M
• Product Overview: Purified recombinant protein of mouse full-length RING1 and YY1 binding protein (Rybp), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites. Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3.
• Molecular Mass: 24.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rybp RING1 and YY1 binding protein [ Mus musculus (house mouse) ]
• Official Symbol: Rybp
• Synonyms: RYBP; RING1 and YY1 binding protein; RING1 and YY1-binding protein; DED-associated factor; Ring1 interactor Rybp; death effector domain-associated factor; DEDAF; YEAF1; 2410018J24Rik; MGC62213
• Gene ID: 56353
• mRNA Refseq: NM_019743
• Protein Refseq: NP_062717
• UniProt ID: Q8CCI5