Recombinant Human RYBP Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RYBP-2901H
• Product Overview: RYBP MS Standard C13 and N15-labeled recombinant protein (NP_036366) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites. Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA.
• Molecular Mass: 24.9 kDa
• AA Sequence: MTMGDKKSPTRPKRQAKPATDEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RYBP RING1 and YY1 binding protein [ Homo sapiens (human) ]
• Official Symbol: RYBP
• Synonyms: RYBP; RING1 and YY1 binding protein; RING1 and YY1-binding protein; AAP1; apoptin associating protein 1; death effector domain associated factor; DEDAF; ring1 interactor RYBP; YEAF1; YY1 and E4TF1 associated factor 1; APAP-1; DED-associated factor; apoptin-associating protein 1; YY1 and E4TF1-associated factor 1; death effector domain-associated factor
• Gene ID: 23429
• mRNA Refseq: NM_012234
• Protein Refseq: NP_036366
• MIM: 607535
• UniProt ID: Q8N488