Recombinant Mouse Ring1 Protein, Myc/DDK-tagged

• Cat.No.: Ring1-5519M
• Product Overview: Purified recombinant protein of mouse full-length ring finger protein 1 (Ring1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
• Molecular Mass: 39.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ring1 ring finger protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ring1
• Synonyms: RING1; ring finger protein 1; E3 ubiquitin-protein ligase RING1; polycomb complex protein RING1; transcription repressor Ring1A; Ring1A; MGC91250
• Gene ID: 19763
• mRNA Refseq: NM_009066
• Protein Refseq: NP_033092
• UniProt ID: O35730