Recombinant Mouse RHOB Protein

Source :	Mammalian Cells
Species :	Mouse
Tag :	His
Form :	Liquid or lyophilized powder
Endotoxin :	< 1.0 EU per μg of the protein as determined by the LAL method.
Purity :	>80%
Notes :	This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Storage :	Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Storage Buffer :	PBS buffer

Gene Name	Rhob ras homolog gene family, member B [ Mus musculus ]
Official Symbol	RHOB
Gene ID	11852
mRNA Refseq	NM_007483.2
Protein Refseq	NP_031509.1
MIM
UniProt ID	P62746

Case 1: Yang J, et al. Microbiome. 2022

The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed. RhoB regulates multiple cell functions; however, its role in colitis is unexplored. In this study, RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis. Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation. Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway. Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.

Fig1. Immunohistochemistry for RhoB in the colon from C57BL/6J mice.

Fig2. Body weight loss of WT and RhoB+/−/RhoB−/− mice after DSS treatment.

Case 2: Englert M, et al. Blood Adv. 2022

Rho GTPases play important roles in platelet biogenesis and function. RhoA-deficient mice display macrothrombocytopenia and a striking mislocalization of megakaryocytes into bone marrow sinusoids and a specific defect in G-protein signaling in platelets. However, the role of the closely related protein RhoB in megakaryocytes or platelets remains unknown. In this study, in contrast to RhoA deficiency, genetic ablation of RhoB in mice results in microthrombocytopenia (decreased platelet count and size). RhoB-deficient platelets displayed mild functional defects predominantly upon induction of the collagen/glycoprotein VI pathway. Megakaryocyte maturation and localization within the bone marrow, as well as actin dynamics, were not affected in the absence of RhoB. However, in vitro-generated proplatelets revealed pronouncedly impaired microtubule organization. Furthermore, RhoB-deficient platelets and megakaryocytes displayed selective defects in microtubule dynamics/stability, correlating with reduced levels of acetylated α-tubulin.

Fig1. Aggregation of RhoB−/− platelets in vitro.

Fig2. Spreading and F-actin dynamics in RhoB−/− platelets.

The mouse RhoB protein is a member of the Ras homologous gene family and GTPase, which regulates intracellular signaling pathways by interacting with signaling pathways such as epidermal growth factor receptor (EGFR), Ras, and phosphatidylinositol 3-kinase (PI3K) /Akt, thereby influencing the response of cell structure and function. Its application is mainly concentrated in the following aspects:

1. Cell function research. As a member of the Rho GTPase family, RhoB protein is involved in the regulation of various intracellular signaling pathways, and the study of RhoB is helpful to further understand the cell signal transduction mechanism. RhoB is involved in the transport of intracellular proteins, such as the transport of EGF receptors from late endosomes to lysosomes, which is of great significance for the study of intracellular transport mechanisms. RhoB plays a role in the cell division phase of the cell cycle and is of great value for the study of cell division.

2. Drug reagent development. Due to its role in tumor development, RhoB may become a target for tumor therapy. Developing inhibitors or drugs that target RhoB may help treat related cancers. The development of RhoB antibodies has been critical for experimental techniques such as Western Blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC/IF), and immunoprecipitation (IP) to detect and study the expression and localization of RhoB protein.

3. Oncology research. RhoB is highly expressed in a variety of malignant tumors and is closely related to the occurrence, invasion and metastasis of tumors. Studying the function of RhoB in tumor is helpful to understand the invasion and metastasis mechanism of tumor.