Recombinant Mouse Rasgrp1 Protein, Myc/DDK-tagged

• Cat.No.: Rasgrp1-5389M
• Product Overview: Purified recombinant protein of mouse full-length RAS guanyl releasing protein 1 (Rasgrp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways. Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses. May also function in differentiation of other cell types. Proto-oncogene, which promotes T-cell lymphomagenesis when its expression is deregulated.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 90.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rasgrp1 RAS guanyl releasing protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Rasgrp1
• Synonyms: RASGRP1; RAS guanyl releasing protein 1; RAS guanyl-releasing protein 1; calDAG-GEFII; calcium and DAG-regulated guanine nucleotide exchange factor II; Rasgrp
• Gene ID: 19419
• mRNA Refseq: NM_011246
• Protein Refseq: NP_035376
• UniProt ID: Q9Z1S3