Recombinant Mouse Rapgef3 Protein, Myc/DDK-tagged

• Cat.No.: Rapgef3-5377M
• Product Overview: Purified recombinant protein of mouse full-length Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
• Molecular Mass: 99.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rapgef3 Rap guanine nucleotide exchange factor (GEF) 3 [ Mus musculus (house mouse) ]
• Official Symbol: Rapgef3
• Synonyms: RAPGEF3; Rap guanine nucleotide exchange factor (GEF) 3; rap guanine nucleotide exchange factor 3; cAMP-GEFI; exchange protein directly activated by cAMP 1; cAMP-regulated guanine nucleotide exchange factor I; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; Epac; Epac1; 2310016P22Rik; 9330170P05Rik; MGC19192
• Gene ID: 223864
• mRNA Refseq: NM_144850
• Protein Refseq: NP_659099
• UniProt ID: Q8VCC8