Recombinant Mouse Rad54l Protein, Myc/DDK-tagged

• Cat.No.: Rad54l-5352M
• Product Overview: Purified recombinant protein of mouse full-length RAD54 like (S. cerevisiae) (Rad54l), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Plays also an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role in telomere capping. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. LIG4 and RAD54L cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 84.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rad54l RAD54 like (S. cerevisiae) [ Mus musculus (house mouse) ]
• Official Symbol: Rad54l
• Synonyms: RAD54L; RAD54 like (S. cerevisiae); DNA repair and recombination protein RAD54-like; mHR54; mRAD54; RAD54 homolog; RAD54
• Gene ID: 19366
• mRNA Refseq: NM_001122958
• Protein Refseq: NP_001116430
• UniProt ID: P70270