Recombinant Mouse Pus1 Protein, His-tagged

• Cat.No.: Pus1-2007M
• Product Overview: Recombinant Mouse Pus1 Protein (Met1-Asp423) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Met1-Asp423
• Description: Enables chromatin binding activity; nuclear receptor coactivator activity; and tRNA pseudouridine synthase activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II and tRNA pseudouridine synthesis. Located in nucleolus. Part of transcription regulator complex. Used to study myopathy, lactic acidosis, and sideroblastic anemia. Human ortholog(s) of this gene implicated in myopathy, lactic acidosis, and sideroblastic anemia 1. Orthologous to human PUS1 (pseudouridine synthase 1).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 51.2 kDa
  Accurate Molecular Mass: 53 kDa
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Pus1 pseudouridine synthase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pus1
• Synonyms: PUS1; pseudouridine synthase 1; tRNA pseudouridine synthase A, mitochondrial; tRNA-uridine isomerase I; tRNA pseudouridylate synthase I; tRNA pseudouridine(38-40) synthase; MPUS1; mPus1p; A730013B20Rik
• Gene ID: 56361
• mRNA Refseq: NM_001025561
• Protein Refseq: NP_001020732
• UniProt ID: Q9WU56