Recombinant Full Length Human pseudouridylate synthase 1 Protein, Flag tagged

• Cat.No.: PUS1-34HFL
• Product Overview: Recombinant protein of human pseudouridylate synthase 1 (PUS1), transcript variant 2 with C-Flag tag was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 1-427 aa
• Description: This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.
• Tag: C-Flag
• Molecular Mass: 44.2 kDa
• AA Sequence: MAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Concentration: >0.05 μg/μL as determined by microplate BCA method

Gene Information

• Gene Name: PUS1 pseudouridylate synthase 1 [ Homo sapiens ]
• Official Symbol: PUS1
• Synonyms: PUS1; pseudouridylate synthase 1; tRNA pseudouridine synthase A, mitochondrial; tRNA uridine isomerase I; tRNA-uridine isomerase I; tRNA pseudouridylate synthase I; tRNA pseudouridine(38-40) synthase; mitochondrial tRNA pseudouridine synthase A; MLASA1; MGC11268
• Gene ID: 80324
• mRNA Refseq: NM_001002019
• Protein Refseq: NP_001002019
• MIM: 608109
• UniProt ID: Q9Y606