Recombinant Mouse Prag1 Protein, Myc/DDK-tagged

• Cat.No.: Prag1-5094M
• Product Overview: Purified recombinant protein of mouse full-length PEAK1 related kinase activating pseudokinase 1 (Prag1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth. Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism. Acts as a critical coactivator of Notch signaling.
• Molecular Mass: 148.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Prag1 PEAK1 related kinase activating pseudokinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Prag1
• Synonyms: Prag1; PEAK1 related kinase activating pseudokinase 1; NA; NACK; Sgk223; D8Ertd82; D8Ertd82e; mFLJ00269; 9830148H23; inactive tyrosine-protein kinase PRAG1; notch activation complex kinase; pragmin; sugen kinase 223; tyrosine-protein kinase PRAG1; tyrosine-protein kinase SgK223; EC 2.7.10.2
• Gene ID: 244418
• mRNA Refseq: NM_172911
• Protein Refseq: NP_766499
• UniProt ID: Q571I4