Recombinant Mouse Ppm1d Protein, Myc/DDK-tagged

• Cat.No.: Ppm1d-5046M
• Product Overview: Purified recombinant protein of mouse full-length protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 74.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ppm1d protein phosphatase 1D magnesium-dependent, delta isoform [ Mus musculus (house mouse) ]
• Official Symbol: Ppm1d
• Synonyms: Ppm1d; protein phosphatase 1D magnesium-dependent, delta isoform; Wi; Wip1; AV338790; protein phosphatase 1D; PP2C-delta; p53-induced protein phosphatase 1; protein phosphatase magnesium-dependent 1 delta; EC 3.1.3.16
• Gene ID: 53892
• mRNA Refseq: NM_016910
• Protein Refseq: NP_058606
• UniProt ID: Q9QZ67