Recombinant Mouse Pot1a Protein, MYC/DDK-tagged

• Cat.No.: Pot1a-174M
• Product Overview: Purified recombinant protein of full-length mouse protection of telomeres 1A (Pot1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 71.3 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pot1a protection of telomeres 1A [ Mus musculus (house mouse) ]
• Official Symbol: Pot1a
• Synonyms: Pot1a; protection of telomeres 1A; Pot1; AI851169; 1500031H18Rik; protection of telomeres protein 1; POT1-like telomere end-binding protein
• Gene ID: 101185
• mRNA Refseq: NM_133931
• Protein Refseq: NP_598692
• UniProt ID: Q91WC1