Recombinant Mouse Polr2m Protein, Myc/DDK-tagged

• Cat.No.: Polr2m-5003M
• Product Overview: Purified recombinant protein of mouse full-length polymerase (RNA) II (DNA directed) polypeptide M (Polr2m), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Isoform 1 appears to be stable component of the Pol II(G) complex form of RNA polymerase II. Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in Mediator-dependent regulation of transcription activation. Isoform 1 acts in vitro as negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 41.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Polr2m polymerase (RNA) II (DNA directed) polypeptide M [ Mus musculus (house mouse) ]
• Official Symbol: Polr2m
• Synonyms: POLR2M; polymerase (RNA) II (DNA directed) polypeptide M; DNA-directed RNA polymerase II subunit GRINL1A; protein GRINL1A; glutamate receptor-like protein 1A; DNA-directed RNA polymerase II subunit M; glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A; Grinl1a; AA407243; AA408799; AI303746; AW553238; D9Wsu138e; mKIAA4185; KIAA4185
• Gene ID: 28015
• mRNA Refseq: NM_001164793
• Protein Refseq: NP_001158265
• UniProt ID: Q6P6I6