Recombinant Mouse Pnpla2 Protein, Myc/DDK-tagged

• Cat.No.: Pnpla2-4972M
• Product Overview: Purified recombinant protein of mouse full-length patatin-like phospholipase domain containing 2 (cDNA clone MGC:29206 IMAGE:5027544), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone. Also has acylglycerol transacylase activity. Acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 75.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pnpla2 patatin-like phospholipase domain containing 2 [ Mus musculus (house mouse) ]
• Official Symbol: Pnpla2
• Synonyms: Pnpla2; patatin-like phospholipase domain containing 2; At; Atgl; desn; TTS-2.2; 0610039C21Rik; 1110001C14Rik; patatin-like phospholipase domain-containing protein 2; adipose triglyceride lipase; desnutrin; transport-secretion protein; triglyceride hydrolase; EC 3.1.1.3
• Gene ID: 66853
• mRNA Refseq: NM_025802
• Protein Refseq: NP_080078
• UniProt ID: Q8BJ56

Reviews

05/14/2020

Quick and precise protein expression, a valuable asset.

09/09/2019

Exceptional protein purification, highly recommended for our research.

03/01/2018

Dependable mass spectrometry analysis, enhances our proteomics research.

Q&As

How does PNPLA2 influence energy homeostasis and fatty acid oxidation in muscle tissues?

PNPLA2 influences energy homeostasis in muscles, facilitating fatty acid oxidation for energy production.

How do alterations in PNPLA2 expression or activity affect cardiovascular health and the risk of atherosclerosis?

Changes in PNPLA2 activity affect cardiovascular health, potentially influencing the development of atherosclerosis.

How does PNPLA2 contribute to the regulation of hepatic lipid metabolism and the development of fatty liver disease?

PNPLA2 regulates liver lipid metabolism, impacting the development of fatty liver disease.

What is the impact of PNPLA2 mutations on the pathogenesis of lipid storage diseases?

Mutations in PNPLA2 can lead to lipid storage diseases by disrupting normal lipid metabolism.

What role does PNPLA2 play in the development and progression of obesity and related metabolic disorders?

PNPLA2 plays a significant role in obesity and metabolic syndrome, affecting lipid storage and energy balance.

What are the mechanisms by which PNPLA2 modulates inflammation and insulin sensitivity?

PNPLA2 modulates inflammation and insulin sensitivity, influencing metabolic health and the risk of diabetes.

How does PNPLA2 regulate lipid metabolism and triglyceride hydrolysis in adipose tissue?

PNPLA2 is crucial for lipid metabolism, breaking down triglycerides in adipose tissue for energy use.