Recombinant Mouse Pitrm1 Protein, Myc/DDK-tagged

• Cat.No.: Pitrm1-4883M
• Product Overview: Purified recombinant protein of mouse full-length pitrilysin metallepetidase 1 (Pitrm1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides. It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion. It is a highly efficient protease, at least toward amyloid-beta protein 40. Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently.
• Molecular Mass: 117.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pitrm1 pitrilysin metallepetidase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pitrm1
• Synonyms: PITRM1; pitrilysin metallepetidase 1; presequence protease, mitochondrial; pitrilysin metalloprotease 1; pitrilysin metalloproteinase 1; nuclear transplantation upregulated protein 1; MP1; MP-1; PreP; Ntup1; AA410010; mKIAA1104; 2310012C15Rik
• Gene ID: 69617
• mRNA Refseq: NM_145131
• Protein Refseq: NP_660113
• UniProt ID: Q8K411