Recombinant Mouse Picalm Protein, Myc/DDK-tagged

• Cat.No.: Picalm-4852M
• Product Overview: Purified recombinant protein of mouse full-length phosphatidylinositol binding clathrin assembly protein (cDNA clone MGC:19382 IMAGE:2651109), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).
• Molecular Mass: 123.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Picalm phosphatidylinositol binding clathrin assembly protein [ Mus musculus (house mouse) ]
• Official Symbol: Picalm
• Synonyms: Picalm; phosphatidylinositol binding clathrin assembly protein; fit; CALM; CLTH; fit-; fit1; fit-1; PAP180; mKIAA4114; phosphatidylinositol-binding clathrin assembly protein; clathrin assembly lymphoid myeloid leukemia
• Gene ID: 233489
• mRNA Refseq: NM_146194
• Protein Refseq: NP_666306
• UniProt ID: Q7M6Y3