Recombinant Mouse PDCD1 protein, hFc-tagged

• Cat.No.: PDCD1-0616M
• Product Overview: Recombinant Mouse PDCD1 protein(NP_032824)(Leu25-Gln167), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Leu25-Gln167
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-1, hFc Tag with an affinity constant of 0.62 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Pdcd1 programmed cell death 1 [ Mus musculus ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; mPD-1; protein PD-1; PD-1; Pdc1; Ly101
• Gene ID: 18566
• mRNA Refseq: NM_008798
• Protein Refseq: NP_032824