Active Recombinant Mouse PDCD1 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: PDCD1-370M
• Product Overview: Recombinant Mouse PDCD1 Protein, Fc & Avi-tagged, Biotinylated is expressed from human HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_032824). Predicted N-terminus: Leu 25. This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 44.6 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc&Avi
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag at 10 μg/mL (100 μl/well) can bind Recombinant Mouse PDCD1 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.15-2.5 μg/mL (QC tested).
• Molecular Mass: 44.6 kDa
• Protein length: Leu 25 - Gln 167
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by reduced SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Pdcd1 programmed cell death 1 [ Mus musculus ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; mPD-1; protein PD-1; PD-1; Pdc1; Ly101
• Gene ID: 18566
• mRNA Refseq: NM_008798
• Protein Refseq: NP_032824