Recombinant Human PDCD1 Protein, His-Tagged

• Cat.No.: PDCD1-02H
• Product Overview: Recombinant Human PDCD1 Protein, His-Tagged was expressed in HEK293 cell

Specification

• Description: Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: Recombinant protein has a calculated molecular weight of about 16 kDa. Due to the abundant glycosylation, it migrates as approximately 30-40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
• Endotoxin: Not determined
• Purity: >95%
• Applications: Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
• Usage: This product is furnished for LABORATORY RESEARCH USE ONLY.Not for diagnostic or therapeutic use.
• Storage: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
• Concentration: Determined by Pierce BCA protein assay kit
• Storage Buffer: Lyophilized from a 0.2 µm filtered solution in PBS
• Preservative: None
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
• Shipping: Ice packs
• Purity determined by: SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
• Purification: His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
• Expressed Region: Pro21-Val170

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens (human) ]
• Official Symbol: PDCD1
• Synonyms: Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279), Programmed cell death protein 1, Protein PD-1, hPD-1, Programmed Cell Death 1, Systemic Lupus Erythematosus Susceptibility 2, HPD-1, PD1, Programmed Cell Death 1 Protein, CD279 Antigen, CD279, HPD-L, HSLE1, SLEB2, PD-1
• Gene ID: 5133
• mRNA Refseq: NM_005018.3
• Protein Refseq: NP_005009.2
• MIM: 600244
• UniProt ID: Q15116