Recombinant Mouse Parp3 Protein, Myc/DDK-tagged

• Cat.No.: Parp3-4679M
• Product Overview: Purified recombinant protein of mouse full-length poly (ADP-ribose) polymerase family, member 3 (Parp3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Cooperates with the XRRC6-XRCC5 (Ku70-Ku80) heterodimer to limit end-resection thereby promoting accurate NHEJ Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as XRRC5 and XRCC6. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks. Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 59.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Parp3 poly (ADP-ribose) polymerase family, member 3 [ Mus musculus (house mouse) ]
• Official Symbol: Parp3
• Synonyms: PARP3; poly (ADP-ribose) polymerase family, member 3; poly [ADP-ribose] polymerase 3; NAD+ ADP-ribosyltransferase 3; Poly[ADP-ribose] synthetase-3; ADP-ribosyltransferase (NAD+, poly (ADP-ribose polymerase)-like 3; ADP-ribosyltransferase (NAD+; poly (ADP-ribose polymerase)-like 3; A DP-ribosyltransferase (NAD+; Adprt3; PARP-3; Adprtl3; AW990611; pADPRT-3; A930002C11Rik
• Gene ID: 235587
• mRNA Refseq: NM_145619
• Protein Refseq: NP_663594
• UniProt ID: Q3UGL7