Active Recombinant Human PARP3, HA-tagged

• Cat.No.: PARP3-454H
• Product Overview: Human full-length PARP-3 [ARTD3] is fused to a HA-tag and a His-tag.

Specification

• Description: PARP-3 [ARTD3] is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.
• Source: Baculovirus infected Sf21 Cells
• Species: Human
• Tag: HA
• Form: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol.
• Bio-activity: ≥10U/mg protein (only monoADP-ribosyl transferase activity).
• Purity: ≥98% (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -80°C.
• Storage: Short Term Storage: -20°C; Long Term Storage: -80°C. After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
• Concentration: Lot dependent (0.2-1mg/ml)

Gene Information

• Gene Name: PARP3 poly (ADP-ribose) polymerase family, member 3 [ Homo sapiens ]
• Official Symbol: PARP3
• Synonyms: PARP3; poly (ADP-ribose) polymerase family, member 3; ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) like 3 , ADPRTL3; poly [ADP-ribose] polymerase 3; ADPRT3; hPARP 3; IRT1; NAD+ ADP ribosyltransferase 3; pADPRT 3; poly(ADP ribose) polymerase 3; poly(ADP ribose) synthetase 3; ADPRT-3; poly[ADP-ribose] synthase 3; NAD+ ADP-ribosyltransferase 3; poly(ADP-ribose) synthetase-3; poly[ADP-ribose] synthetase 3; NAD(+) ADP-ribosyltransferase 3; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2; poly (ADP-ribose) polymerase)-like 3; ADPRTL2; ADPRTL3; PADPRT-3
• Gene ID: 10039
• mRNA Refseq: NM_001003931
• Protein Refseq: NP_001003931
• MIM: 607726
• UniProt ID: Q9Y6F1
• Chromosome Location: 3p22.2-p21.1
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem
• Function: NAD+ ADP-ribosyltransferase activity; catalytic activity; transferase activity, transferring glycosyl groups