Recombinant Mouse Orc2 Protein, Myc/DDK-tagged

• Cat.No.: Orc2-4599M
• Product Overview: Purified recombinant protein of mouse full-length origin recognition complex, subunit 2 (Orc2), transcript variant a, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.
• Molecular Mass: 65.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Orc2 origin recognition complex, subunit 2 [ Mus musculus (house mouse) ]
• Official Symbol: Orc2
• Synonyms: ORC2; origin recognition complex, subunit 2; origin recognition complex subunit 2; Orc2l; AU041563
• Gene ID: 18393
• mRNA Refseq: NM_008765
• Protein Refseq: NP_032791
• UniProt ID: Q60862