Recombinant Mouse Ocln Protein, His-tagged

• Cat.No.: Ocln-1882M
• Product Overview: Recombinant Mouse Ocln Protein (Lys264-Pro521) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable protein domain specific binding activity. Involved in negative regulation of gene expression and tight junction organization. Located in several cellular components, including apical plasma membrane; apicolateral plasma membrane; and bicellular tight junction. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in Crohn's disease and pseudo-TORCH syndrome 1. Orthologous to human OCLN (occludin).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.8 kDa
• Protein length: Lys264-Pro521
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Ocln occludin [ Mus musculus (house mouse) ]
• Official Symbol: Ocln
• Synonyms: OCLN; occludin; Ocl; AI503564
• Gene ID: 18260
• mRNA Refseq: NM_008756
• Protein Refseq: NP_032782
• UniProt ID: Q61146