Recombinant Mouse Nudt15 Protein, Myc/DDK-tagged

• Cat.No.: Nudt15-4536M
• Product Overview: Purified recombinant protein of mouse full-length nudix (nucleoside diphosphate linked moiety X)-type motif 15 (Nudt15), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP. Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives. Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear. Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs. May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA.
• Molecular Mass: 19.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nudt15 nudix (nucleoside diphosphate linked moiety X)-type motif 15 [ Mus musculus (house mouse) ]
• Official Symbol: Nudt15
• Synonyms: Nudt15; nudix (nucleoside diphosphate linked moiety X)-type motif 15; MTH2; 6530403O17; A730068G11Rik; nucleotide triphosphate diphosphatase NUDT15; 7,8-dihydro-8-oxoguanine-triphosphatase NUDT15; 8-oxo-dGTPase NUDT15; MutT homolog 2; nucleoside diphosphate-linked moiety X motif 15; nucleoside diphosphate-linked to another moiety X hydrolase 15; nudix hydrolase 15; nudix motif 15; probable 7,8-dihydro-8-oxoguanine triphosphatase NUDT15; probable 8-oxo-dGTP diphosphatase NUDT15; EC 3.6.1.9
• Gene ID: 214254
• mRNA Refseq: NM_172527
• Protein Refseq: NP_766115
• UniProt ID: Q8BG93