Recombinant Mouse Ntmt1 Protein, Myc/DDK-tagged

• Cat.No.: Ntmt1-4520M
• Product Overview: Purified recombinant protein of mouse full-length N-terminal Xaa-Pro-Lys N-methyltransferase 1 (Ntmt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 25.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ntmt1 N-terminal Xaa-Pro-Lys N-methyltransferase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ntmt1
• Synonyms: Ntmt1; N-terminal Xaa-Pro-Lys N-methyltransferase 1; NTM1A; Mettl1; AL033331; AL033332; Mettl11a; 2610205E22Rik; N-terminal Xaa-Pro-Lys N-methyltransferase 1; X-Pro-Lys N-terminal protein methyltransferase 1A; alpha N-terminal protein methyltransferase 1A; methyltransferase like 11A; methyltransferase-like protein 11A; EC 2.1.1.244
• Gene ID: 66617
• mRNA Refseq: NM_170592
• Protein Refseq: NP_733480
• UniProt ID: Q8R2U4