Recombinant Mouse Nt5e protein, His-tagged

• Cat.No.: Nt5e-2302M
• Product Overview: Recombinant Mouse Nt5e protein(Q61503)(Trp29-Lys549), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Trp29-Lys549
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD73, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Nt5e 5 nucleotidase, ecto [ Mus musculus ]
• Official Symbol: Nt5e
• Synonyms: NT5E; 5 nucleotidase, ecto; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik
• Gene ID: 23959
• mRNA Refseq: NM_011851
• Protein Refseq: NP_035981