Recombinant Cynomolgus NT5E protein, His-tagged

• Cat.No.: NT5E-5633C
• Product Overview: Recombinant Cynomolgus NT5E protein(EHH53214.1)(Trp27-Cys554), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Trp27-Cys554
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus CD73, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.