Active Recombinant Mouse Nt5e, His-tagged

Cat.No. : Nt5e-250M
Product Overview : Recombinant Mouse Nt5e (Trp29-Lys549) fused with 6­His tag at C­terminal, was expressed in CHO Cells.
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Description : 5''-nucleotidase(5''-NT), also known as ecto-5''-nucleotidaseor CD73 (Cluster ofDifferentiation 73), is an enzymethat in humans is encoded by theNT5E gene. CD73 commonly serves to convert AMP toadenosine.
Source : CHO Cells
Species : Mouse
Tag : His
Predicted N Terminal : Trp29
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Bio-activity : Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is >10,000 pmol/min/µg.
Molecular Mass : 59 kDa
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
Protein length : 29-549 a.a.
Gene Name Nt5e 5'' nucleotidase, ecto [ Mus musculus (house mouse) ]
Official Symbol Nt5e
Synonyms Nt5e; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik; 5'' nucleotidase, ecto; 5''-nucleotidase; 5''-NT; ecto-5''-nucleotidase; EC 3.1.3.5
Gene ID 23959
mRNA Refseq NM_011851
Protein Refseq NP_035981
UniProt ID Q61503
Chromosome Location 9; 9 E3.2
Pathway Metabolism of nucleotides; Nicotinate and nicotinamide metabolism; Purine metabolism
Function 5''-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds

Not For Human Consumption!

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