Recombinant Mouse Nr1h3 Protein, Myc/DDK-tagged

• Cat.No.: Nr1h3-4486M
• Product Overview: Purified recombinant protein of mouse full-length nuclear receptor subfamily 1, group H, member 3 (Nr1h3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators.
• Molecular Mass: 50.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nr1h3 nuclear receptor subfamily 1, group H, member 3 [ Mus musculus (house mouse) ]
• Official Symbol: Nr1h3
• Synonyms: NR1H3; nuclear receptor subfamily 1, group H, member 3; oxysterols receptor LXR-alpha; LXR alpha; liver X receptor alpha; ubiquitously-expressed nuclear receptor 1; LXR; RLD1; Unr1; AU018371
• Gene ID: 22259
• mRNA Refseq: NM_013839
• Protein Refseq: NP_038867
• UniProt ID: Q9Z0Y9