Recombinant Mouse Ngly1 Protein, Myc/DDK-tagged

• Cat.No.: Ngly1-4409M
• Product Overview: Purified recombinant protein of mouse full-length N-glycanase 1 (Ngly1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.
• Molecular Mass: 74.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ngly1 N-glycanase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ngly1
• Synonyms: Ngly1; N-glycanase 1; Pn; Png1; PNGase; 1110002C09Rik; peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; peptide N-glycanase homolog; EC 3.5.1.52
• Gene ID: 59007
• mRNA Refseq: NM_021504
• Protein Refseq: NP_067479
• UniProt ID: Q9JI78