Recombinant Mouse Nek6 Protein, Myc/DDK-tagged

• Cat.No.: Nek6-4368M
• Product Overview: Purified recombinant protein of mouse full-length NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Phosphorylates STAT3.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 35.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nek6 NIMA (never in mitosis gene a)-related expressed kinase 6 [ Mus musculus (house mouse) ]
• Official Symbol: Nek6
• Synonyms: NEK6; NIMA (never in mitosis gene a)-related expressed kinase 6; serine/threonine-protein kinase Nek6; nimA-related protein kinase 6; NIMA-related expressed kinase 6; never in mitosis A-related kinase 6; 1300007C09Rik
• Gene ID: 59126
• mRNA Refseq: NM_001159631
• Protein Refseq: NP_001153103
• UniProt ID: Q9ES70